信息查询
 时时热点
普通文章 2017年度两优一先… (7月14日)
普通文章 基础所院党委举办… (7月14日)
普通文章 中国医学科学院基… (6月20日)热点文章
普通文章 2017年公开招聘启… (6月16日)热点文章
普通文章 中国医学科学院基… (6月15日)
普通文章 张学教授荣获首届… (5月27日)
普通文章 科学与奉献精神的… (5月16日)
普通文章 基础所院机关党支… (5月4日)热点文章
普通文章 2017年公开招聘启… (4月27日)热点文章
普通文章 院校师生追思缅怀… (4月12日)热点文章
 最新调查
 没有任何调查
 友情链接
院校直属:
其它部门:
Capture and Ligation Probe-PCR (CLIP-PCR) for Molecular Screening, with Application to Active Malaria Surveillance for Elimination.
[ 作者:科技处    来源自:本站原创    点击数:16020    更新时间:2015-5-19    文章编辑:wangluo ]

减小字体 增大字体

Cheng Z1, Wang D2, Tian X1, Sun Y1, Sun X3, Xiao N4, Zheng Z5.

Clin Chem. 2015 May 11. pii: clinchem.2014.237115. PMID: 25964304

Abstract
BACKGROUND:
Malaria control programs have achieved remarkable success during the past decade. Nonetheless, sensitive and affordable methods for active screening of malaria parasites in low-transmission settings remain urgently needed.
METHODS:
We developed a molecular screening method, capture and ligation probe-PCR (CLIP-PCR), which achieved the sensitivity of reverse-transcription PCR but eliminated the reliance on RNA purification and reverse transcription. In this method, 18S rRNA of genus Plasmodium is released from blood, captured onto 96-well plates, and quantified by the amount of ligated probes that bind continuously to it. We first used laboratory-prepared samples to test the method across a range of parasite densities and pool sizes, then applied the method to an active screening of 3358 dried blood spot samples collected from 3 low-endemic areas in China.
RESULTS:
Plasmodium falciparum diluted in whole blood lysate could be detected at a concentration as low as 0.01 parasites/μL, and a pool size of ≤36 did not significantly affect assay performance. When coupled with a matrix pooling strategy, the assay drastically increased throughput to thousands of samples per run while reducing the assay cost to cents per sample. In the active screening, CLIP-PCR identified 14 infections, including 4 asymptomatic ones, with <500 tests, costing <US$0.60 for each sample. All positive results were confirmed by standard quantitative PCR.
CONCLUSIONS:
CLIP-PCR, by use of dried blood spots with a pooling strategy, efficiently offers a highly sensitive and high-throughput approach to detect asymptomatic submicroscopic infections with reduced cost and labor, making it an ideal tool for large-scale malaria surveillance in elimination settings.

 上一篇文章: A paper-based skin patch for the diagnostic screening of cystic fibrosis.
 下一篇文章: Quantitative survey of multiple CpGs from 5 genes identifies CpG methylation panel discriminating between high- and low-grade cervical intraepithelial neoplasia
打印此文】【关闭窗口
CopyRight © 2009 中国医学科学院基础医学研究所&北京协和医学院基础医学院 All Rights Reserved  
地址:北京市东城区东单三条五号   信箱:bgs@ibms.pumc.edu.cn    邮编:100730